ZEISS Webinar

Introduction to Super-resolution Microscopy

ZEISS Elyra 7 with Lattice SIM²

Tue, 29 August 2023

10:30 AM - 12:00 PM IST

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SIM Apotome : Flexible Optical Sectioning

Using SIM² Apotome in Elyra 7

The SIM Apotome acquisition mode in combination with the SIM² reconstruction algorithm now allows you to further tune the gentleness of your fast live-cell imaging with high contrast and resolution. You can use your new optical sectioning speed to increase your productivity when acquiring large sample areas or large volumes at different magnifications.

In this talk we will explore the possibilities of in-depth imaging of live samples using SIM² Apotome mode which will give fast optical sectioning with crisp contrast and high lateral and axial resolution.

Researchers interested in SR imaging at a resolution of 60nm with high temporal resolution of 255fps without compromising on depth of field should attend this webinar.

  
      Lattice SIM - 3D volume imaging using Elyra 7
      
    Neurons in a mouse brain

ZEISS Webinar Agenda

Learn More About Elyra 7

Webinar Highlights

Explore the concept of going beyond the diffraction limit in light microscopy
Demonstration of doing multi-color Super-resolution imaging for conventionally stained samples
How can one accelerate image acquisition in all three dimensions
Expand your possibilities with Single Molecule Localization Microscopy
How to do live cell imaging at SR with no photobleaching

Webinar Schedule

Date- 15th June, 2023

Time- 10:30 AM - 12:00 PM IST

Speaker's Profile Somenath Ghatak Product Application & Sales Specialist, LM Life Science

Dr. Somenath Ghatak obtained his Ph.D in Biophysics from AIIMS, New Delhi and has more than 10 years of experience in application driven research microscopy. He has been supporting users in all aspects of advanced light microscopy as well as correlative microscopy experiments: sample preparation, introduction to the imaging technology, assistance and trouble-shooting during imaging and data collection, data management as well as data analysis.

Application Videos

ZEISS Elyra 7

Elyra 7 -Leap Mode
Elyra 7 Defining Specific Needs for Speed and Resolution
Elyra 7 Fast Tile Scanning of Very Large Areas

Elyra 7 Leap mode accelerates the volume imaging speed three times and at the same time decreases the light dosage on your sample. While still capturing all the finest details, the entire volume (18 planes) of the U2OS cell expressing Calreticulin-tdTomato was imaged at 38 volumes / min speed in Lattice SIM acquisition mode. For SIM Apotome acquisition mode, you can expect up to three times higher volume imaging speed.

Elyra 7 Leap Mode - Three Times Faster Digital Sectioning
The need for higher imaging speeds and decreased light dosage is almost unlimited. The robustness of Elyra 7 structured illumination patterns plus the image reconstruction software allow a significant reduction to the number of phase images required for both Lattice SIM and SIM Apotome acquisition modes, while decreasing the resolution only slightly. Lattice SIM acquisition can be operated at 9 phase images per frame while 3 phase images per frame are sufficient for SIM Apotome, increasing the imaging speed by 44 % and 66 %, respectively.

Elyra 7 Defining Specific Needs for Speed and Resolution
The high-speed performance of SIM Apotome acquisition mode allows for fast tile scan imaging of very large areas at excellent sectioning quality. A mulberry section of 11.1 mm² × 11 µm size was imaged using Nyquist sampling in all three directions and in two colors in less than 2 minutes. Similar speeds also were achieved for a cross section of a leaf.

Elyra 7 Fast Tile Scanning of Very Large Areas

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